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What is the principle of spectrophotometer? What are the applications?
Release time:
2021-11-12 11:03
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Spectrophotometers are instruments based on absorption spectroscopy used to analyze and determine the concentration or composition of chemical substances in solutions or gases. The principle is basically to use the principle that the amount of natural light absorbed by the compound is proportional to its concentration. In spectrophotometry, monochromatic light is emitted from the visible or ultraviolet region, and the amount of light absorbed by the sample is measured, thereby calculating the concentration of the compound in the sample.
The core technology of the spectrophotometer is the spectrometer. The spectrometer is a key component of the instrument. Its function is to change the visible light or ultraviolet light into a beam of monochromatic light through the cutter, and then through the compound immersion liquid in the specimen room, and then measure the amount of light absorbed by the compound. Since the absorption spectrum of each compound has its absorption peak, the concentration of the compound in the sample can be determined according to the position and size of the absorption peak.
Photometer is widely used in quantitative analysis, quality control and scientific research.
1, biochemical analysis: can be used to determine the content of biological substances, such as protein, lipid and DNA. In the medical field, it can be used to determine the liver, kidney and heart and other medical examination indicators, such as bilirubin, urea and creatinine.
2, water quality testing: can be used to determine the content of chemical substances in water, such as nutrients, organic compounds and metal ions. These chemicals can interfere with the use of drinking water, making it no longer safe or usable.
3, drug analysis: can be used for drug analysis, has an important application in drug quality control. It is possible to check whether the concentration of the components of the drug meets the prescribed standards.
4, environmental analysis: can be used to determine the content of pollutants in air, soil and water. In environmental monitoring, it is often used to analyze the content of harmful gases in the atmosphere, such as hydrogen sulfide, sulfur dioxide and nitrogen oxides.
In conclusion, spectrophotometry is an accurate and reliable scientific tool that can be widely used in biology, chemistry, medicine, environmental science, and other fields. The technology has a wide range of applications and can be used in areas such as quantitative analysis, quality control and research. Through use, we can obtain information about the absorption peaks of the sample, thereby understanding the chemical substances and their contents in the culture and environment.
The core technology of the spectrophotometer is the spectrometer. The spectrometer is a key component of the instrument. Its function is to change the visible light or ultraviolet light into a beam of monochromatic light through the cutter, and then through the compound immersion liquid in the specimen room, and then measure the amount of light absorbed by the compound. Since the absorption spectrum of each compound has its absorption peak, the concentration of the compound in the sample can be determined according to the position and size of the absorption peak.
Photometer is widely used in quantitative analysis, quality control and scientific research.
1, biochemical analysis: can be used to determine the content of biological substances, such as protein, lipid and DNA. In the medical field, it can be used to determine the liver, kidney and heart and other medical examination indicators, such as bilirubin, urea and creatinine.
2, water quality testing: can be used to determine the content of chemical substances in water, such as nutrients, organic compounds and metal ions. These chemicals can interfere with the use of drinking water, making it no longer safe or usable.
3, drug analysis: can be used for drug analysis, has an important application in drug quality control. It is possible to check whether the concentration of the components of the drug meets the prescribed standards.
4, environmental analysis: can be used to determine the content of pollutants in air, soil and water. In environmental monitoring, it is often used to analyze the content of harmful gases in the atmosphere, such as hydrogen sulfide, sulfur dioxide and nitrogen oxides.
In conclusion, spectrophotometry is an accurate and reliable scientific tool that can be widely used in biology, chemistry, medicine, environmental science, and other fields. The technology has a wide range of applications and can be used in areas such as quantitative analysis, quality control and research. Through use, we can obtain information about the absorption peaks of the sample, thereby understanding the chemical substances and their contents in the culture and environment.
Spectrophotometer
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